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SRP68 antibody

Anti-SRP68 antibody used in Western Blot (WB). GTX03442
Anti-SRP68 antibody used in IHC (Paraffin sections) (IHC-P). GTX03442
Anti-SRP68 antibody used in IHC (Paraffin sections) (IHC-P). GTX03442
Anti-SRP68 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03442
Anti-SRP68 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03442
Anti-SRP68 antibody used in IHC (Paraffin sections) (IHC-P). GTX03442
Anti-SRP68 antibody used in IHC (Paraffin sections) (IHC-P). GTX03442
Anti-SRP68 antibody used in Western Blot (WB). GTX03442
Anti-SRP68 antibody used in IHC (Paraffin sections) (IHC-P). GTX03442

Cat. No. GTX03442

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SRP68 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human SRP68(Accession Q9UHB9), corresponding to amino acid residues S566-K587.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal recognition particle 68

Cellular Localization

Cytoplasm , Nucleolus

Background

This gene encodes a subunit of the signal recognition particle (SRP). The SRP is a ribonucleoprotein complex that transports secreted and membrane proteins to the endoplasmic reticulum for processing. The complex includes a 7S RNA and six protein subunits. The encoded protein is the 68kDa component of the SRP, and forms a heterodimer with the 72kDa subunit that is required for SRP function. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and three pseudogenes of this gene are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, May 2012]

Database

DATA IMAGES

Anti-SRP68 antibody used in Western Blot (WB). GTX03442

GTX03442 WB Image

WB analysis of human kidney tissue lysate using GTX03442 SRP68 antibody. The lane on the left was treated with blocking peptide.

Anti-SRP68 antibody used in IHC (Paraffin sections) (IHC-P). GTX03442

GTX03442 IHC-P Image

IHC-P analysis of rat brain tissue using GTX03442 SRP68 antibody antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-SRP68 antibody used in IHC (Paraffin sections) (IHC-P). GTX03442

GTX03442 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX03442 SRP68 antibody antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-SRP68 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03442

GTX03442 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX03442 SRP68 antibody.
Permeabilization : 0.1% Triton X-100
Red : Primary antibody
Green : beta tubulin
Blue : DAPI
Dilution : 1:200

Anti-SRP68 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03442

GTX03442 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX03442 SRP68 antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-SRP68 antibody used in IHC (Paraffin sections) (IHC-P). GTX03442

GTX03442 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX03442 SRP68 antibody antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-SRP68 antibody used in IHC (Paraffin sections) (IHC-P). GTX03442

GTX03442 IHC-P Image

IHC-P analysis of rat heart tissue using GTX03442 SRP68 antibody antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-SRP68 antibody used in Western Blot (WB). GTX03442

GTX03442 WB Image

WB analysis of various sample lysates using GTX03442 SRP68 antibody.
Lane 1 : Mouse brain tissue lysate treated with blocking peptide
Lane 2 : Mouse brain tissue lysate
Lane 3 : 293 whole cell lysate
Lane 4 : Hybridoma cell lysates

Anti-SRP68 antibody used in IHC (Paraffin sections) (IHC-P). GTX03442

GTX03442 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX03442 SRP68 antibody antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399